Diversity, phylogeny and pathogenicity of Botryosphaeriaceae on nonnative Eucalyptus grown in an urban environment: A case study

《Diversity, phylogeny and pathogenicity of Botryosphaeriaceae on nonnative Eucalyptus grown in an urban environment: A case study》

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来源: URBAN FORESTRY & URBAN GREENING,Vol.26,P.139-148

语言: 英文

关键字: Die-back; Endophytes; Latent pathogens; Pathogenicity; Urban habitat; NATIVE SYZYGIUM-CORDATUM; SOUTH-AFRICA; SP-NOV; PLANTATION EUCALYPTUS; WESTERN-AUSTRALIA; 1ST REPORT; CANKER; TREES; DISEASES; COMPLEX

作者单位: [Pavlic-Zupanc, Draginja; Maleme, Happy M.; Wingfield, Michael J.] Univ Pretoria, Dept Microbiol & Plant Pathol, Private Bag X20, ZA-0028 Pretoria, South Africa. [Wingfield, Brenda D.; Slippers, Bernard] Univ Pretoria, Dept Genet, DST NRF Ctr Excellence Tree Hlth Biotechnol CTHB, Forestry & Agr Biotechnol Inst,Fac Nat & Agr Sci, Private Bag X20, ZA-0028 Pretoria, South Africa. [Piskur, Barbara] Slovenian Forestry Inst, Dept Forest Protect, Vecna Pot 2, SI-1000 Ljubljana, Slovenia. Pavlic-Zupanc, D (reprint author), Univ Pretoria, Dept Microbiol & Plant Pathol, Private Bag X20, ZA-0028 Pretoria, South Africa. E-Mail: pavlicdr@gmail.com

摘要: The Botryosphaeriaceae are opportunistic pathogens mostly of woody plants, including Eucalyptus. These fungi can cause cankers and die-back diseases on non-native Eucalyptus trees in South African plantations. Botryosphaeriaceae were isolated from diseased and asymptomatic twigs and leaves from 20 Eucalyptus spp. grown in a Pretoria, South Africa arboretum and its surroundings. The isolates were initially grouped based on conidial morphology and Internal Transcribed Spacer (ITS) rDNA PCR-RFLP profiles. They were further identified using DNA sequence data for the ITS rDNA and translation elongation factor 1-alpha (TEF-1 alpha) gene regions and tested for pathogenicity. Five species were identified including Botryosphaeria dothidea and four Neofusicoccum species namely Neofusicoccum parvum; N. cryptoaustrale and N. ursorum that were recently described from plant tissues collected as a part of the current study; and Neofusicoccum eucalypti (Winter) Maleme, Pavlic & Slippers comb. nov. The latter species is recorded for the first time on Eucalyptus in South Africa. Most of the identified species were collected from the leaves of 17 different Eucalyptus spp. Neofusicoccum parvum was most commonly isolated (72% of all isolates) followed by B. dothidea species complex (17%). With exception of N. parvum which was isolated from majority of Eucalyptus spp. the other species were isolated from limited number of Eucalyptus species indicating host-preferences. All the isolated Botryosphaeriaceae species produced lesions on inoculated Eucalyptus grandis plants that were significantly larger than those associated with the controls.